Wide variety of top suppliers highquality customer support. Plasmid pcmv sportbgal is used as a positive control for monitoring expression in eukaryotic cells. This website uses cookies to ensure you get the best experience. Snapgene viewer is a versatile tool for creating and sharing richly annotated sequence files. Transcripts from this locus can be induced or inhibited by cell stretch and affect cell differentiation in the myogenic or adipogenic pathways. The plasmid also contains a cmv promoter upstream of the bgal gene, followed by the sv40. Expressionready cdna clones mgc premier expressionready cdna clones for human, mouse, rat and bovine genomes. A mutation in the low voltagegated calcium channel. The ha epitope tag is wellcharacterized and highly immunoreactive. Vector information pcmv ha vector information pt32835 sold as part of cat.
Gateway cloning technology the wolfson centre for applied. An mlui site is introduced upon ligation of a cdna insert. The pcmv ha mammalian expression vector expresses proteins containing an nterminal hemagglutinin ha epitope tag. The celera cdna clone collection from life technologies contains both public clones and proprietary clones that are available only from life technologies. Addgene continues to monitor the changing covid19 situation. The cdna was polished with t4 polymerase, digested with not i to create 5blunt3not i cdna, then sizefractionated on a gel, purified, and ligated into the pcmv sport6. Lhx29 and etv1 transcription factors have complementary.
We have resumed shipping of plasmid orders in a reduced capacity. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a. Pdia6 promotes the proliferation of hela cells a hela cells were transfected with plasmid pcmv sport6 pdia6 or empty vector pcmv sport6 and subjected to colony formation assay. By continuing to use this site, you agree to the use of cookies. Gateway technology enables rapid transfer of genes into multiple gene expression systems see page 3. Gateway cloning technology instruction manual, but does not provide rights to.
If you have pending orders, we will email you to confirm that your organization can accept shipments prior to shipping your order. We notice that you are using internet explorer, which is not supported by xenbase and may cause the site to display incorrectly. Pdf we developed various binary vectors that can be used for expressing a foreign gene in rice. To see this sequence with restriction sites, features, and translations, please download.
Expressionready vector options combined with the highest quality fulllength cdna content provide the fastest path to a successful experiment. B pdia6 overexpression increased the colony number compared to negative control. Virion stiffness regulates immature hiv1 entry core. Transgenicdrak2overexpressioninmiceleadstoincreased. This locus encodes a member of the methyltransferase family, and is involved in chromatin remodeling. This plasmid also contains a portion of the 3 utr of hmcgr1. The encoded dgat2 protein has the flag epitope at the amino terminus.
The pcramplified dna product was digested with the restriction enzymes sali and noti and ligated into the pcmv sport6 vector. Because of the size of the cmr gene, the coding sequence of cmr was directionally cloned in two pieces into pcmv sport6. For further information, please contact uirf at 3193354546. Pdia6 promotes the proliferation of hela cells through. Correlation between hepatitis b virus protein and microrna. Available downloads the following download material is available for vector pcmvsport6. The plasmid contains the reporter gene bgalactosidase bgal from e.
Using custom cdna library introduction custom cdna library in pcmv sport6. The major differences in pcmv sport vectors are around the multiple cloning site. Image mgc premier expressionready cloning vector pcmv sport6 empty vector, tcc0gvo. We are committed to providing customers with innovative research tools and helping determine the mechanisms underlying cell function and diseases. It functions as a transcription factor that regulates transcription of specific target genes. The orientation and sequence of the cgr coding sequence were then veri. Prior to january 2001, life technologies, invitrogen corp. Lhx29 and etv1 may regulate the levels and boundaries of slit1sema3a expression. Research paper pdia6 promotes the proliferation of hela.
Terms and conditions 20042018 harvard medical school plasmid was created and is maintained by the dfhcc dna resource core at harvard medical schooldfhcc dna resource core at harvard medical school. A hela cells were transfected with plasmid pcmv sport6 pdia6 or empty vector pcmv sport6 and subjected to colony formation assay. An expression plasmid pcmv sport6 flagmmgat1 encoding a flag epitopetagged version of the mouse mgat1 protein was constructed as follows. Analysis of the resulting sequences indicates that more than 70% of the clones contain fulllength cdna inserts. A fulllength infectious cdna clone of zika virus from. The fulllength human cdna clone collection contains.
This is a free resource for the scientific community that is compiled by addgene this page is informational only this vector is not available from addgene please contact the manufacturer for further details. Custom cdna library in pcmv sport6 thermo fisher scientific. Welcome to vector database vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Dm pegtels lab contains the insert cd63phluorin and is published in j cell biol. Gateway library vector for cloning and transient mammalian cell expression of cdnas. Cloning of chicken mineralocorticoid receptor for transient transfection. The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation.
Nox3 regulation by noxo1, p47phox, and p67phox received for publication, january 20, 2004, and in revised form, may 10, 2004. Supplementary information materials and methods figs. Because there should be no barriers to seeing your data. See pcmv sport6 for an older version of this vector.
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